# Speaker notes / talking points

[Atlas] 166,420 esophageal cells, seven states, EoE versus healthy.
[Composition] Mast cells expand about 14-fold; epithelium rebalances.
[Mast states] Disease-associated states carry SIGLEC6 — 84% in EoE.
[Epithelial] Basal-zone expansion and shifted differentiation.
[HLA-I] Induction concentrates in suprabasal epithelium and mast cells.
[Signaling] HLA and S100 signaling rewire across states.
[Eosinophils] They don't survive dissociation, so they sit in our bulk streams.
[Next] Map the presented peptides and connect to pMHC work.