# Speaker notes / talking points

[Background] EoE and celiac are both antigen-driven diseases where food proteins cross a defective barrier and get presented via MHC to T cells -- EoE via CD4/Th2, celiac via HLA-DQ-restricted gluten response. [Hypothesis] We tested whether a single pMHC pipeline, run across both diseases, finds shared food antigens and transferable design lessons. [Methods] Same antigen-to-panel pipeline, with celiac adding a TG2-deamidation step and a unique benchmark against 31 known Sollid epitopes. [Landscape] Class II dominates: DRB1*01:01 carries 925 of 1800 strong binder predictions; class I is sparse (9/372 strong) and secondary. [Shared load] Milk and egg antigens are jointly presented in both diseases, with class-II binder density around 10x class-I. [Engineering] Protease-hardening cut cleavage sites by 45-49% in two candidate binders, aimed at gut-stable reagents. [Diagnostics] Milk and soy dominate the diagnostic peptide-pool sizes (720 and 526 strong epitopes). [Ask] We're seeking collaborators for functional validation (tetramer/AIM assays) of top-ranked DQ2.5/DRB1 peptides and feedback on the shared-antigen diagnostic pool design.